Journal: American Journal of Cancer Research
Article Title: Immuno-PET imaging of VEGFR-2 expression in prostate cancer with 89 Zr-labeled ramucirumab
doi:
Figure Lengend Snippet: The VEGFR-2 expression in prostate cancer (PrCa) cell lines measured by flow cytometry. The percentage of cell counts are from the same gate of top positive cells in fluorescent intensity in the three PrCa cell lines. Sample groups: Anti-r 2nd Ab only, the controls engaging with Alexa488 anti-rabbit secondary antibody only; Anti-h 2nd Ab only, the controls engaging with Alexa488 anti-human secondary antibody only; 55B11 as 1st Ab, the samples engaging with rabbit anti-human 55B11 as the primary antibody; Df-R as 1st Ab, the samples engaging with deferoxamine-conjugated Ramucirumab as the primary antibody (for PC-3 cell line, n = 4; for LAPC-4 and LNCAP cell lines, n = 3).
Article Snippet: X.0.7; Tree Star, Inc.; Ashland, OR), shift values of the top 1% of the positive counts in fluorescent intensity were gated on the histograms of LAPC-4 blank cells as the baseline control.
Techniques: Expressing, Flow Cytometry